Activity of DNA-dependent RNA polymerases in rabbit mammary gland during lactogenesis
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چکیده
منابع مشابه
Diet-induced obesity impairs mammary development and lactogenesis in murine mammary gland.
We have developed a mouse model of diet-induced obesity that shows numerous abnormalities relating to mammary gland function. Animals ate approximately 40% more calories when offered a high-fat diet and gained weight at three times the rate of controls. They exhibited reduced conception rates, increased peripartum pup mortality, and impaired lactogenesis. The impairment of lactogenesis involved...
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The DNA-dependent RNA polymerases of members of the three orders of methanogens were purified and their enzymatic properties described. The enzymes consist of 7-8 polypeptides. Although these differed in molecular mass, the four heaviest components could be allied to components of the enzyme of Methanobacterium thermoautotrophicum, W by cross-reaction with antibodies directed against the denatu...
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DNA-dependent RNA polymerases of immature and castrated rat uteri were studied after estradiol administration. The enzymes were solubilized from either whole uterus homogenate or nuclei and their activities were measured on an exogenous DNA template. oc-Amanitin was used to distinguish Lu-amanitin-resistant from cu-amanitin-senitive forms of the enzyme. The number of oc-amanitin-sensitive RNA p...
متن کاملEffect of Estradiol on Rat Uterus DNA-dependent RNA Polymerases
A systematic approach to the in vitro measurement of HNA-dependent RNA polymerase activities in nuclei from immature rat uterus has been undertaken in order to evaluate the early effects of estradiol. The experimental conditions include excess of nucleoside .i’-triphosphate and short time assays (6 min) at low temperature (25”) in order to obtain the maximal velocity of the reaction and to mini...
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A bimodal change in yield and microsomal protein content of rabbit mammary gland was observed with the progress of pregnancy and lactation. The initial stimulus took place on day 22 of pregnancy and the second during early lactation. Palmitoyl-CoA:monopalmitoyl-sn-glycerol 3-phosphate palmitoyltransferase activity was monitored concurrently. This enzyme in rabbit mammary microsomes is composed ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80149-x